Comparing the Effect of Aerobic Exercise with Different Intensities on FNDC۵ Protein Level of Muscle Tissue in Obese Wistar Rats

Introduction: A recent over expression model demonstrated that peroxisome proliferator-activated receptor coactivator ۱-alpha (PGC-۱α) regulates the expression of fibronectin type III domain containing protein ۵ (FNDC۵); a novel myokine with a potential role in stimulating brown-fat-like development in white adipose tissue. The aim of this study was to investigate the effects of eight weeks of aerobic training with different intensities on FNDC۵ protein in soleus muscle tissue of obese male Wistar rats. Methods: ۲۴ adult rats (weight: ۲۵۰ to ۳۰۰ gr, BMI >۳۰g/cm۲) were divided into three groups: aerobic training with ۷۰ to ۷۵% Vo۲max (moderate intensity), aerobic training with ۸۰ to ۸۵% Vo۲max (high intensity) and, control group. All training groups carried out exercise training for ۸ weeks running on treadmill (۵ sessions/per-week for ۶۰ min per session). After the training period, the level of FNDC۵ protein was measured. Statistical test of ANOVA was used for data analysis to determine the difference between groups and post hoc test of Tukey was used for paired comparisons (p≤۰.۰۵). Results: The levels of FNDC۵ in soleus muscle tissue in both moderate intensity and high intensity aerobic training group increased significantly (P=۰.۰۰۱). Conclusion: According to the results, increasing of FNDC۵ as a result of eight weeks of moderate and high intensity aerobic training with moderate and high intensity, it seems that FNDC۵ can mediate conversion of white to brown tissue and so affect losing weight and thermogenesis.