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مقالات فارسیISIکنفرانسهاژورنالها

Differential Expression of Circulating miRNA-۱۰۳ in Women with and without Polycystic Ovary Syndrome

محل انتشار: مجله بین المللی آزمایشگاه پزشکی، دوره: 9، شماره: 4
سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 196

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استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:
https://civilica.com/doc/1607520

شناسه ملی سند علمی:

JR_JIML-9-4_006

تاریخ نمایه سازی: 2 اسفند 1401

چکیده مقاله:

Background and Aims: Polycystic ovary syndrome (PCOS) has a largely unknown etiology and is a common heterogeneous endocrine disorder in women of reproductive age, characterized by polycystic ovaries, hyperandrogenism, insulin resistance, and chronic anovulation. MicroRNAs (miRNAs) are small, noncoding RNA sequences that negatively regulate gene expression at the post-transcriptional level. miRNA-۱۰۳ has been associated with metabolic disorders such as obesity and diabetes, which are also associated with PCOS. This study aims to describe the different expressions of circulating miR-۱۰۳ in the plasma of PCOS women. Materials and Methods: This case-control study includes ۲۵ women with PCOS and ۲۵ healthy women. Plasma total RNA was isolated and, after polyadenylation, converted to total cDNA. Then, the expression level of microRNA-۱۰۳ was analyzed by quantitative reverse transcription-polymerase chain reaction, and SNORD miRNA was used as the reference gene. Results: Our results indicate that the expression level of miRNA-۱۰۳ significantly decreased in the PCOS group compared to healthy women, which can be related to the etiology and progression of PCOS (p < ۰.۰۵). Conclusions: Our findings recommend further studies in the larger statistical population and more accurate techniques to confirm microRNA-۱۰۳ as a noninvasive diagnosis biomarker.

کلیدواژه ها:

Biomarker ، MicroRNA-۱۰۳ ، Polycystic ovary syndrome ، Q-PCR

نویسندگان

محمدرضا ناصری

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Biology, Faculty of Science, Science and Art University, Yazd, Iran

سید مهدی کلانتر

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

نرگس نیکونهاد

Department of Biology, Faculty of Science, Science and Art University, Yazd, Iran

هانیه شفیع نیا

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

فاطمه منتظری

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Department of Biology, Faculty of Science, Science and Art University, Yazd, Iran

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