Identification of Methicillin-Resistant Staphylococcus aureus in poultry meat portions using Multiplex PCR

Staphylococcus aureus is a prevalent bacterial colonizer with zoonotic potential, affecting humans and various animal species, including livestock, poultry, and pets. This study investigates methicillin-resistant S. aureus (MRSA) isolates in poultry meat portions using Multiplex PCR methods. A total of ۲۱۰ samples, including ۷۰ wings, ۷۰ thighs, and ۷۰ necks, were collected from Mashhad, Iran markets. S. aureus identification employed culture and phenotypical methods, while the disk diffusion method assessed antibiotic susceptibility using ۱۴ different disks. The Multiplex PCR assay was developed to confirm S. aureus isolates and detect antibiotic resistance genes. Among the ۲۱۰ samples, ۵۲ (۲۴.۷۶%) tested positive for S. aureus. Antibiotic susceptibility testing revealed that ۱۷ (۳۲.۶۹%) of the S. aureus isolates were resistant to methicillin. Tetracycline exhibited the highest resistance, followed by ampicillin (۶۱.۵%) and penicillin (۵۷%). Conversely, chloramphenicol demonstrated the lowest resistance at ۳.۸%. All isolates were susceptible to gentamicin, vancomycin, imipenem, and ciprofloxacin. PCR analysis confirmed the presence of ۱۶S rRNA and femA genes in all isolates, while ۱۴ (۲۶.۹۲%) harbored the methicillin-resistant gene (mecA). The study suggests multiplex PCR is a valuable and sensitive technique for detecting antibiotic resistance genes in S. aureus within chicken meat, emphasizing its utility in surveillance and control efforts